ubiquitylation assay kit Search Results


ubiquitylation assay kit  (Enzo Biochem)
90
Enzo Biochem ubiquitylation assay kit
Ubiquitylation Assay Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ubiquitylation assay kit/product/Enzo Biochem
Average 90 stars, based on 1 article reviews
ubiquitylation assay kit - by Bioz Stars, 2026-03
90/100 stars
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ubiquitylation assay kit  (Cayman Chemical)
90
Cayman Chemical ubiquitylation assay kit
FKB degrades Skp2 protein in a proteasome and ubiquitination dependent manner. a , Western blotting analysis of Myc-Skp2 and endogenous Skp2 protein expression after PC3/Skp2 or PC3/PCDNA cells were treated with FKB, MG132 or their combination for 24 h. b , proteasome activities (i.e. OD values) were measured by the 20S Proteasome Assay Kit after PC3 cells were treated with 0.1% DMSO, FKB or MG132 for 24 h. c , Skp2 ubiquitination was measured by immunoprecipitation with anti-ubiquitin antibody and then Western blotting analysis of immunoprecipitates by anti-Skp2 after PC3 cells were treated with 0.1% DMSO or FKB (left two panels). 293 cells were transiently transfected with Skp2 and ubiquitin expression plasmids. After 24 h of transfection, cells were treated with 0.1% DMSO or FKB for 16 h. FKB increases ubiquitination of Skp2. d , the relative ubiquitination levels were measured by the <t>Ubiquitylation</t> Assay Kit after DU145 cells were treated by 0.1% DMSO, 8.8 μM FKB, 5 μM MG132 for 16 h
Ubiquitylation Assay Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ubiquitylation assay kit/product/Cayman Chemical
Average 90 stars, based on 1 article reviews
ubiquitylation assay kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
kit for the isolation and enrichment of ubiquitylated proteins  (Biomol GmbH)
90
Biomol GmbH kit for the isolation and enrichment of ubiquitylated proteins
FKB degrades Skp2 protein in a proteasome and ubiquitination dependent manner. a , Western blotting analysis of Myc-Skp2 and endogenous Skp2 protein expression after PC3/Skp2 or PC3/PCDNA cells were treated with FKB, MG132 or their combination for 24 h. b , proteasome activities (i.e. OD values) were measured by the 20S Proteasome Assay Kit after PC3 cells were treated with 0.1% DMSO, FKB or MG132 for 24 h. c , Skp2 ubiquitination was measured by immunoprecipitation with anti-ubiquitin antibody and then Western blotting analysis of immunoprecipitates by anti-Skp2 after PC3 cells were treated with 0.1% DMSO or FKB (left two panels). 293 cells were transiently transfected with Skp2 and ubiquitin expression plasmids. After 24 h of transfection, cells were treated with 0.1% DMSO or FKB for 16 h. FKB increases ubiquitination of Skp2. d , the relative ubiquitination levels were measured by the <t>Ubiquitylation</t> Assay Kit after DU145 cells were treated by 0.1% DMSO, 8.8 μM FKB, 5 μM MG132 for 16 h
Kit For The Isolation And Enrichment Of Ubiquitylated Proteins, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kit for the isolation and enrichment of ubiquitylated proteins/product/Biomol GmbH
Average 90 stars, based on 1 article reviews
kit for the isolation and enrichment of ubiquitylated proteins - by Bioz Stars, 2026-03
90/100 stars
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p53 ubiquitylation kit  (Boston Biochem)
90
Boston Biochem p53 ubiquitylation kit
FKB degrades Skp2 protein in a proteasome and ubiquitination dependent manner. a , Western blotting analysis of Myc-Skp2 and endogenous Skp2 protein expression after PC3/Skp2 or PC3/PCDNA cells were treated with FKB, MG132 or their combination for 24 h. b , proteasome activities (i.e. OD values) were measured by the 20S Proteasome Assay Kit after PC3 cells were treated with 0.1% DMSO, FKB or MG132 for 24 h. c , Skp2 ubiquitination was measured by immunoprecipitation with anti-ubiquitin antibody and then Western blotting analysis of immunoprecipitates by anti-Skp2 after PC3 cells were treated with 0.1% DMSO or FKB (left two panels). 293 cells were transiently transfected with Skp2 and ubiquitin expression plasmids. After 24 h of transfection, cells were treated with 0.1% DMSO or FKB for 16 h. FKB increases ubiquitination of Skp2. d , the relative ubiquitination levels were measured by the <t>Ubiquitylation</t> Assay Kit after DU145 cells were treated by 0.1% DMSO, 8.8 μM FKB, 5 μM MG132 for 16 h
P53 Ubiquitylation Kit, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p53 ubiquitylation kit/product/Boston Biochem
Average 90 stars, based on 1 article reviews
p53 ubiquitylation kit - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
pde activity assay kit  (Abcam)
92
Abcam pde activity assay kit
FKB degrades Skp2 protein in a proteasome and ubiquitination dependent manner. a , Western blotting analysis of Myc-Skp2 and endogenous Skp2 protein expression after PC3/Skp2 or PC3/PCDNA cells were treated with FKB, MG132 or their combination for 24 h. b , proteasome activities (i.e. OD values) were measured by the 20S Proteasome Assay Kit after PC3 cells were treated with 0.1% DMSO, FKB or MG132 for 24 h. c , Skp2 ubiquitination was measured by immunoprecipitation with anti-ubiquitin antibody and then Western blotting analysis of immunoprecipitates by anti-Skp2 after PC3 cells were treated with 0.1% DMSO or FKB (left two panels). 293 cells were transiently transfected with Skp2 and ubiquitin expression plasmids. After 24 h of transfection, cells were treated with 0.1% DMSO or FKB for 16 h. FKB increases ubiquitination of Skp2. d , the relative ubiquitination levels were measured by the <t>Ubiquitylation</t> Assay Kit after DU145 cells were treated by 0.1% DMSO, 8.8 μM FKB, 5 μM MG132 for 16 h
Pde Activity Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pde activity assay kit/product/Abcam
Average 92 stars, based on 1 article reviews
pde activity assay kit - by Bioz Stars, 2026-03
92/100 stars
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ubiquitylation assay kit  (Advisains)
99
Advisains ubiquitylation assay kit
FKB degrades Skp2 protein in a proteasome and ubiquitination dependent manner. a , Western blotting analysis of Myc-Skp2 and endogenous Skp2 protein expression after PC3/Skp2 or PC3/PCDNA cells were treated with FKB, MG132 or their combination for 24 h. b , proteasome activities (i.e. OD values) were measured by the 20S Proteasome Assay Kit after PC3 cells were treated with 0.1% DMSO, FKB or MG132 for 24 h. c , Skp2 ubiquitination was measured by immunoprecipitation with anti-ubiquitin antibody and then Western blotting analysis of immunoprecipitates by anti-Skp2 after PC3 cells were treated with 0.1% DMSO or FKB (left two panels). 293 cells were transiently transfected with Skp2 and ubiquitin expression plasmids. After 24 h of transfection, cells were treated with 0.1% DMSO or FKB for 16 h. FKB increases ubiquitination of Skp2. d , the relative ubiquitination levels were measured by the <t>Ubiquitylation</t> Assay Kit after DU145 cells were treated by 0.1% DMSO, 8.8 μM FKB, 5 μM MG132 for 16 h
Ubiquitylation Assay Kit, supplied by Advisains, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ubiquitylation assay kit/product/Advisains
Average 99 stars, based on 1 article reviews
ubiquitylation assay kit - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier

Image Search Results


FKB degrades Skp2 protein in a proteasome and ubiquitination dependent manner. a , Western blotting analysis of Myc-Skp2 and endogenous Skp2 protein expression after PC3/Skp2 or PC3/PCDNA cells were treated with FKB, MG132 or their combination for 24 h. b , proteasome activities (i.e. OD values) were measured by the 20S Proteasome Assay Kit after PC3 cells were treated with 0.1% DMSO, FKB or MG132 for 24 h. c , Skp2 ubiquitination was measured by immunoprecipitation with anti-ubiquitin antibody and then Western blotting analysis of immunoprecipitates by anti-Skp2 after PC3 cells were treated with 0.1% DMSO or FKB (left two panels). 293 cells were transiently transfected with Skp2 and ubiquitin expression plasmids. After 24 h of transfection, cells were treated with 0.1% DMSO or FKB for 16 h. FKB increases ubiquitination of Skp2. d , the relative ubiquitination levels were measured by the Ubiquitylation Assay Kit after DU145 cells were treated by 0.1% DMSO, 8.8 μM FKB, 5 μM MG132 for 16 h

Journal: Cell Communication and Signaling : CCS

Article Title: Flavokawain B targets protein neddylation for enhancing the anti-prostate cancer effect of Bortezomib via Skp2 degradation

doi: 10.1186/s12964-019-0338-2

Figure Lengend Snippet: FKB degrades Skp2 protein in a proteasome and ubiquitination dependent manner. a , Western blotting analysis of Myc-Skp2 and endogenous Skp2 protein expression after PC3/Skp2 or PC3/PCDNA cells were treated with FKB, MG132 or their combination for 24 h. b , proteasome activities (i.e. OD values) were measured by the 20S Proteasome Assay Kit after PC3 cells were treated with 0.1% DMSO, FKB or MG132 for 24 h. c , Skp2 ubiquitination was measured by immunoprecipitation with anti-ubiquitin antibody and then Western blotting analysis of immunoprecipitates by anti-Skp2 after PC3 cells were treated with 0.1% DMSO or FKB (left two panels). 293 cells were transiently transfected with Skp2 and ubiquitin expression plasmids. After 24 h of transfection, cells were treated with 0.1% DMSO or FKB for 16 h. FKB increases ubiquitination of Skp2. d , the relative ubiquitination levels were measured by the Ubiquitylation Assay Kit after DU145 cells were treated by 0.1% DMSO, 8.8 μM FKB, 5 μM MG132 for 16 h

Article Snippet: Ubiquitylation Assay Kit and 20S Proteasome Assay Kit were from Cayman and Abcam, respectively.

Techniques: Ubiquitin Proteomics, Western Blot, Expressing, Immunoprecipitation, Transfection, Ubiquitin Assay